眼针对缺血性脑卒中大鼠认知障碍、炎症因子及微血管密度的影响

Effects of Eye Acupuncture on Cognitive Impairment， Inflammatory Factors， and Microvessel Density in Rats with Ischemic Stroke

摘要:

目的：观察眼针对缺血性脑卒中（IS） 大鼠认知障碍、炎症因子及微血管密度的影响。方法：将60只雄性健康SPF级SD大鼠随机分为对照组、假手术组、模型组、体针组及眼针组，各12只。除对照组及假手术组外均构建IS动物模型，模型制备成功后体针组大鼠针刺百会穴，眼针组大鼠针刺眼周的肝区、肾区、上焦区、下焦区，比较各组大鼠神经功能缺损评分、认知功能、炎症因子、脑组织梗死体积、脑组织病理形态及微血管密度。结果：与对照组、假手术组比较，模型组大鼠神经功能缺损评分升高（P＜0.05）；与模型组比较，体针组及眼针组神经功能缺损评分均降低（P＜0.05），且眼针组低于体针组（P＜0.05）。对照组与假手术组逃避潜伏期、穿过平台次数、白细胞介素-6 （IL-6）、白细胞介素-1β （IL-1β）、肿瘤坏死因子-α（TNF-α）、脑组织CD31阳性细胞数比较，差异均无统计学意义（P＞0.05）；与对照组及假手术组比较，模型组逃避潜伏期延长，穿过平台次数增多，IL-6、IL-1β、TNF-α水平升高，脑组织梗死体积增大，而CD31阳性细胞数降低，差异均有统计学意义（P＜0.05）；与模型组比较，体针组逃避潜伏期缩短，穿过平台次数减少，IL-6、IL-1β、TNF-α 水平降低，脑组织梗死体积减小，CD31 阳性细胞数升高，差异均有统计学意义（P＜0.05）；与体针组比较，眼针组逃避潜伏期更短，穿过平台次数更少，IL-6、IL-1β、TNF-α水平更低，脑组织梗死体积更小，CD31阳性细胞数更高，差异均有统计学意义（P＜0.05）。结论：眼针能有效改善IS大鼠的认知功能与神经功能缺损状态，可通过下调IL-6、IL-1β、TNF-α等炎症因子水平减轻脑组织炎症反应，同时可缩小脑组织梗死体积、提升脑组织微血管密度，对缺血性脑损伤发挥保护作用。

Abstract:

Abstract：Objective：To observe the effects of eye acupuncture on cognitive impairment，inflammatory factors， and microvessel density in rats with ischemic stroke （IS）. Methods：A total of 60 healthy male SPF-grade SD rats were randomly divided into the control group， the sham operation group， the model group， the body acupuncture group， and the eye acupuncture group，with 12 rats in each group. Except for the control group and the sham operation group， rats in the other three groups were used to establish IS models. After modeling，the rats in the body acupuncture group received acupuncture at Baihui （GV20） point， and those in the eye acupuncture group were treated at periocular acupoint regions including the liver region， kidney region， upper energizer region， and lower energizer region. The neurological deficit scores， cognitive function， inflammatory factor levels， cerebral infarct volume， brain tissue pathological changes， and microvessel density were compared among all groups. Results： After intervention， compared with the control group and the sham operation group，the neurological deficit scores in the model group were increased （P＜0.05）， and compared with the model group， the neurological deficit scores in the body acupuncture group and the eye acupuncture group were decreased （P＜0.05）， with the eye acupuncture group presenting lower scores than the body acupuncture group （P＜0.05）. No significant differences were observed in escape latency， platform crossing times，levels of interleukin-6（ IL-6），interleukin-1β（ IL-1β），tumor necrosis factor-α（ TNF-α）， and the number of CD31-positive cells between the control group and the sham operation group （P＞0.05）. Relative to the control group and the sham operation group， the model group had prolonged escape latency， increased platform crossing times， increased levels of IL-6， IL-1β， and TNF- α， enlarged cerebral infarct volume， and reduced number of CD31-positive cells，with all differences statistically significant （P＜0.05）. Compared with the model group， the body acupuncture group showed shortened escape latency， decreased platform crossing times， decreased IL-6， IL-1β， and TNF-α levels and cerebral infarct volume， and increased number of CD31-positive cells （P＜0.05）. Furthermore，the eye acupuncture group achieved shorter escape latency，less platform crossing times，lower IL-6， IL-1β，and TNF-α levels，smaller cerebral infarct volume，and more CD31-positive cells than the body acupuncture group （P＜0.05）. Conclusion： Eye acupuncture can effectively improve cognitive function and relieve neurological deficits in IS rats. It exerts protective effects against ischemic brain injury by down-regulating the levels of inflammatory factors such as IL-6， IL-1β， and TNF- α to alleviate cerebral inflammatory responses， reduce cerebral infarct volume，and enhance cerebral microvessel density.